Senin, 11 Februari 2013

Komposisi media untuk menyemai biji anggrek

Komposisi media untuk menyemai biji anggrek


K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524

Knudson C modified orchid medium
Knudson C orchid medium, morel modification
Phytamax orchid maintenance medium without charcoal
Phytamax orchid medium with charcoal and banana powder
Phytamax orchid maintenance medium
Phytamax orchid multiplication medium
Murashige and Skoog basal salt mixture (MS)
  (listed for purposes of comparison only)


Component (mg/L)
K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524*

Aluminum chloride • 6H2O








Ammonium nitrate

500.0
825.0
825.0
825.0
825.0
1650.0

Ammonium phosphate monobasic








Ammonium sulfate
500.0
500.0






Boric acid
0.056

3.1
3.1
3.1
3.1
6.2

Calcium chloride anhydrous


166.0
166.0
166.0
166.0
332.2

Calcium nitrate
694.4
347.2






Calcium phosphate tribasic








Cobalt chloride • 6H2O


0.0125
0.0125
0.0125
0.0125
0.025

Cupric sulfate • 5H2O
0.0624

0.0125
0.0125
0.0125
0.0125
0.025

Na2-EDTA


37.24
37.24
37.24
37.24
37.26

ZnNa2-EDTA








Ferric chloride • 6H2O








Ferric citrate








Ferric sulfate








Ferric tartrate • 2H2O








Ferrous sulfate • 7H2O
25.0
25.0
27.85
27.85
27.85
27.85
27.8

Magnesium sulfate
122.125
122.125
90.35
90.35
90.35
90.35
180.7

Manganese chloride • 4H2O








Manganese sulfate • H2O
5.682
5.682
8.45
8.45
8.45
8.45
16.9

Molybdenum trioxide
0.016







Molybdic acid (sodium salt) • 2H2O


0.125
0.125
0.125
0.125
0.25

Nickel chloride • 6H2O








Nickel sulfate • 6H2O








Potassium chloride

250.0






Potassium iodide


0.415
0.415
0.415
0.415
0.83

Potassium nitrate


950.0
950.0
950.0
950.0
1900.0

Potassium phosphate monobasic
250.0
250.0
85.0
85.0
85.0
85.0
170.0

Potassium sulfate








Sodium nitrate








Sodium phosphate monobasic








Sodium sulfate








Zinc nitrate • 6H2O








Zinc sulfate • 7H2O
0.331

5.3
5.3
5.3
5.3
8.6

Organics (mg/L)
K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524*

Activated charcoal



2000.0
2000.0



Adenine hemisulfate








Agar








Banana powder (50% malto-dextrin)



30




6-Benzylaminopurine (BA)





2.0


D-Biotin








6gamma-gamma-
Dimethylallylaminopurine








Glycine (free base)








Indole-3-acetic acid








Indole-3-butyric acid








Kinetin








MES (free acid)


1000.0
1000.0
1000.0
1000.0


myo-Inositol


100.0
100.0
100.0
100.0


alpha-Naphthaleneacetic acid





0.5


Nictotinic acid (free acid)


1.0
1.0
1.0
0.5


Peptone


2000.0
2000.0
2000.0
2000.0


Pyridoxine • HCl


1.0
1.0
1.0
0.5


Sucrose
20
20
20
20
20
20


Thiamine • HCl


10.0
10.0
10.0
1.0



K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524*

Grams of powder to prepare 1 L
21.6
22.0
25.3
57.3
27.3
25.3
4.3

pH ± 0.5 at room temperature
4.7
4.8
5.0
5.2
5.4
5.0
3.9

*Composition for M 5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of


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