Komposisi media untuk menyemai biji anggrek
K 4003 K 4128 P 0931 P 1056 P 6668 P 6793 M 5524 |
Knudson C modified orchid medium Knudson C orchid medium, morel modification Phytamax orchid maintenance medium without charcoal Phytamax orchid medium with charcoal and banana powder Phytamax orchid maintenance medium Phytamax orchid multiplication medium Murashige and Skoog basal salt mixture (MS) (listed for purposes of comparison only) |
Component (mg/L)
|
K
4003
|
K
4128
|
P
0931
|
P
1056
|
P
6668
|
P
6793
|
M
5524*
|
|
Aluminum chloride • 6H2O
|
|
|
|
|
|
|
|
|
Ammonium nitrate
|
|
500.0
|
825.0
|
825.0
|
825.0
|
825.0
|
1650.0
|
|
Ammonium phosphate monobasic
|
|
|
|
|
|
|
|
|
Ammonium sulfate
|
500.0
|
500.0
|
|
|
|
|
|
|
Boric acid
|
0.056
|
|
3.1
|
3.1
|
3.1
|
3.1
|
6.2
|
|
Calcium chloride anhydrous
|
|
|
166.0
|
166.0
|
166.0
|
166.0
|
332.2
|
|
Calcium nitrate
|
694.4
|
347.2
|
|
|
|
|
|
|
Calcium phosphate tribasic
|
|
|
|
|
|
|
|
|
Cobalt chloride • 6H2O
|
|
|
0.0125
|
0.0125
|
0.0125
|
0.0125
|
0.025
|
|
Cupric sulfate • 5H2O
|
0.0624
|
|
0.0125
|
0.0125
|
0.0125
|
0.0125
|
0.025
|
|
Na2-EDTA
|
|
|
37.24
|
37.24
|
37.24
|
37.24
|
37.26
|
|
ZnNa2-EDTA
|
|
|
|
|
|
|
|
|
Ferric chloride • 6H2O
|
|
|
|
|
|
|
|
|
Ferric citrate
|
|
|
|
|
|
|
|
|
Ferric sulfate
|
|
|
|
|
|
|
|
|
Ferric tartrate • 2H2O
|
|
|
|
|
|
|
|
|
Ferrous sulfate • 7H2O
|
25.0
|
25.0
|
27.85
|
27.85
|
27.85
|
27.85
|
27.8
|
|
Magnesium sulfate
|
122.125
|
122.125
|
90.35
|
90.35
|
90.35
|
90.35
|
180.7
|
|
Manganese chloride • 4H2O
|
|
|
|
|
|
|
|
|
Manganese sulfate • H2O
|
5.682
|
5.682
|
8.45
|
8.45
|
8.45
|
8.45
|
16.9
|
|
Molybdenum trioxide
|
0.016
|
|
|
|
|
|
|
|
Molybdic acid (sodium salt) • 2H2O
|
|
|
0.125
|
0.125
|
0.125
|
0.125
|
0.25
|
|
Nickel chloride • 6H2O
|
|
|
|
|
|
|
|
|
Nickel sulfate • 6H2O
|
|
|
|
|
|
|
|
|
Potassium chloride
|
|
250.0
|
|
|
|
|
|
|
Potassium iodide
|
|
|
0.415
|
0.415
|
0.415
|
0.415
|
0.83
|
|
Potassium nitrate
|
|
|
950.0
|
950.0
|
950.0
|
950.0
|
1900.0
|
|
Potassium phosphate monobasic
|
250.0
|
250.0
|
85.0
|
85.0
|
85.0
|
85.0
|
170.0
|
|
Potassium sulfate
|
|
|
|
|
|
|
|
|
Sodium nitrate
|
|
|
|
|
|
|
|
|
Sodium phosphate monobasic
|
|
|
|
|
|
|
|
|
Sodium sulfate
|
|
|
|
|
|
|
|
|
Zinc nitrate • 6H2O
|
|
|
|
|
|
|
|
|
Zinc sulfate • 7H2O
|
0.331
|
|
5.3
|
5.3
|
5.3
|
5.3
|
8.6
|
|
Organics (mg/L)
|
K
4003
|
K
4128
|
P
0931
|
P
1056
|
P
6668
|
P
6793
|
M
5524*
|
|
Activated charcoal
|
|
|
|
2000.0
|
2000.0
|
|
|
|
Adenine hemisulfate
|
|
|
|
|
|
|
|
|
Agar
|
|
|
|
|
|
|
|
|
Banana powder (50% malto-dextrin)
|
|
|
|
30
|
|
|
|
|
6-Benzylaminopurine (BA)
|
|
|
|
|
|
2.0
|
|
|
D-Biotin
|
|
|
|
|
|
|
|
|
6gamma-gamma-
Dimethylallylaminopurine
|
|
|
|
|
|
|
|
|
Glycine (free base)
|
|
|
|
|
|
|
|
|
Indole-3-acetic acid
|
|
|
|
|
|
|
|
|
Indole-3-butyric acid
|
|
|
|
|
|
|
|
|
Kinetin
|
|
|
|
|
|
|
|
|
MES (free acid)
|
|
|
1000.0
|
1000.0
|
1000.0
|
1000.0
|
|
|
myo-Inositol
|
|
|
100.0
|
100.0
|
100.0
|
100.0
|
|
|
alpha-Naphthaleneacetic acid
|
|
|
|
|
|
0.5
|
|
|
Nictotinic acid (free acid)
|
|
|
1.0
|
1.0
|
1.0
|
0.5
|
|
|
Peptone
|
|
|
2000.0
|
2000.0
|
2000.0
|
2000.0
|
|
|
Pyridoxine • HCl
|
|
|
1.0
|
1.0
|
1.0
|
0.5
|
|
|
Sucrose
|
20
|
20
|
20
|
20
|
20
|
20
|
|
|
Thiamine • HCl
|
|
|
10.0
|
10.0
|
10.0
|
1.0
|
|
|
|
K
4003
|
K
4128
|
P
0931
|
P
1056
|
P
6668
|
P
6793
|
M
5524*
|
|
Grams of powder to prepare 1 L
|
21.6
|
22.0
|
25.3
|
57.3
|
27.3
|
25.3
|
4.3
|
|
pH ± 0.5 at room temperature
|
4.7
|
4.8
|
5.0
|
5.2
|
5.4
|
5.0
|
3.9
|
|
*Composition for
M 5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of
|
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